Chiu, Vaughan receive NIH Transformative Research Award

Assistant Professor Joshua Vaughan and Professor Daniel Chiu are among the eight awardees selected by the National Institutes of Health (NIH) for a 2017 NIH Director’s Transformative Research Award. This program is one of four in the NIH High-Risk, High-Reward Research program, which funded 86 awards to scientists working in biomedical research in 2017.

The NIH High-Risk, High-Reward Research program funds exceptionally creative scientists proposing to use highly innovative approaches to tackle major challenges in biomedical research. The program accelerates scientific discovery by supporting high-risk ideas with high-impact potential, and applicants are encouraged to think outside the box and to pursue exciting, trailblazing ideas in any area of research relevant to the NIH mission.

The NIH Director’s Transformative Research Award, established in 2009, promotes cross-cutting, interdisciplinary approaches and is open to individuals and teams of investigators who propose research that could potentially create or challenge existing paradigms.

Professors Chiu and Vaughan are developing radical new technologies for high-resolution mapping of brain tissue, including circuit-level spatial information down to a resolution of 50 nanometers and comprehensive analysis of the types of proteins present across large regions of the brain. These techniques are needed because it is technically difficult to directly detect large numbers of proteins in brain tissue.

Instead of trying to measure proteins directly, most approaches measure RNA molecules—a precursor to proteins. But RNA detection in spatially complex brain tissue has its flaws. Current approaches struggle with dim signals that are difficult to detect over background noise in complex, thick tissues. Professors Chiu and Vaughan will develop new fluorescent probes to light up RNA molecules in tissues and will use a novel, large-area light sheet microscope—together with sample processing techniques—to rapidly probe large volumes of brain tissue at high spatial resolution.

The work of 2017 NIH award recipients will be featured at the 2018 High-Risk, High-Reward Research Symposium, held June 6-8, 2018 in Bethesda, MD. The symposium is free, open to the public, and will bring together recipients of the NIH Director’s Pioneer, New Innovator, Transformative Research, and Early Independence awards to share their groundbreaking research and discoveries.

To learn more about Professor Chiu and his research, please visit his faculty page and research group website.

To learn more about Professor Vaughan and his research, please visit his faculty page and research group website.

Adapted by Diana Knight from a October 6, 2017 story by Jennifer Langston of UW News and Leila Gray of UW Health Sciences/UW Medicine.

UW and PNNL launch NW IMPACT in joint pursuit of new materials

UW News story by James Urton

Many innovations of 21st century life, from touch screens and electric cars to fiber-optics and implantable devices, grew out of research on new materials. This impact of materials science on today’s world has prompted two of the leading research institutions in the Pacific Northwest to join forces to research and develop new materials that will significantly influence tomorrow’s world.

With this eye toward the future, the Department of Energy’s Pacific Northwest National Laboratory and the University of Washington announced the creation of the Northwest Institute for Materials Physics, Chemistry and Technology — or NW IMPACT — a joint research endeavor to power discoveries and advancements in materials that transform energy, telecommunications, medicine, information technology and other fields. UW President Ana Mari Cauce and PNNL Director Steven Ashby formally launched NW IMPACT during a ceremony on January 31 at the PNNL campus in Richland, WA.

PNNL and UW leaders attend the launch of NW IMPACT at the PNNL campus on Jan. 31. Left-to-right: NW IMPACT co-director David Ginger; UW President Ana Mari Cauce; PNNL Director Steven Ashby; NW IMPACT co-director Jim De Yoreo.

PNNL and UW leaders attend the launch of NW IMPACT at the PNNL campus on Jan. 31. Left-to-right: NW IMPACT co-director David Ginger; UW President Ana Mari Cauce; PNNL Director Steven Ashby; NW IMPACT co-director Jim De Yoreo. Andrea Starr/PNNL

“This partnership holds enormous potential for innovations in materials science that could lead to major changes in our lives and the world,” said Cauce. “We are excited to strengthen the ties between our two organizations, which bring complementary strengths and a shared passion for ground-breaking discovery.”

“The science of making new materials is vital to a wide range of advancements, many of which we have yet to imagine,” said Ashby. “By combining ideas, talent and resources, I have no doubt our two organizations will find new ways to improve lives and provide our next generation of materials scientists with valuable research opportunities.”

The institute builds on a history of successful partnerships between the UW and PNNL, including joint faculty appointments and past collaborations such as the Materials Synthesis and Simulations Across Scales Initiative, the PNNL-led Battery 500 consortium, and the UW Molecular Engineering Materials Center, a new NSF-funded Materials Research Science and Engineering (MRSEC) Center. But NW IMPACT is the beginning of a long-term partnership, forging deeper ties between the UW and PNNL.

The goal is to leverage these respective strengths to enable discoveries, innovations and educational opportunities that would not have been possible by either institution alone.

UW President Ana Mari Cauce speaking with Miqin Zhang, a UW professor of materials science and engineering, at the launch of NW IMPACT on Jan. 31.

UW President Ana Mari Cauce speaking with Miqin Zhang, a UW professor of materials science and engineering, at the launch of NW IMPACT on Jan. 31. Andrea Starr/PNNL

“By partnering the UW and PNNL together through NW IMPACT, the sum will truly be greater than the parts,” said David Ginger, a UW professor of chemistry and chief scientist at the UW Clean Energy Institute. “We are joining together our expertise and experiences to create the next generation of leaders who will create the materials of the future.”

Ginger will co-lead the institution in its initial phase with Jim De Yoreo, chief scientist for materials synthesis and simulation across scales at PNNL and a joint appointee at the UW.

Over its first few years, NW IMPACT aims to hire a permanent institute director, who will be based at both PNNL and the UW; create at least 20 new joint UW-PNNL appointments among existing researchers; streamline access to research facilities at the UW’s Seattle campus and PNNL’s Richland campus for institute projects; involve at least 20 new UW graduate students in PNNL-UW collaborations; and provide seed grants to institute-affiliated researchers to tackle new scientific frontiers in a collaborative fashion.

Some of the areas in which NW IMPACT will initially focus include:

  • Materials for energy conversion and storage, which can be applied to more efficient solar cells, batteries and industrial applications. These include innovative approaches to create flexible, ultrathin solar cells for buildings or fabrics, long-lasting batteries for implantable medical devices, catalysts to enable high efficiency energy conversion and industrial processes, and manufacturing methods to synthesize these materials efficiently for commercial applications.
  • Quantum materials, such as ultrathin semiconductors or other materials that can harness the rules of quantum mechanics at subatomic-level precision for applications in quantum computing, telecommunications and beyond.
  • Materials for water separation and utilization, which include processes to make water purification and ocean desalination methods faster, cheaper and more energy-efficient.
  • Biomimetic materials, which are synthetic materials inspired by the structures and design principles of biological molecules and materials within our cells — including proteins and DNA. These materials could be applicable in medical settings for implantable devices or tissue engineering, and for self-assembled protein-like scaffolds in industrial settings.

“The science of making materials involves understanding where the atoms must be placed in order to obtain the properties needed for specific applications, and then understanding how to get the atoms where they need to be,” said De Yoreo.

NW IMPACT will draw on the unique strengths and talents of each institution for innovative collaborations in these areas. For example, PNNL has broad expertise in materials for improved batteries. The lab also offers best-in-class imaging, NMR and mass spectrometry capabilities at EMSL, the Environmental Molecular Sciences Laboratory, a DOE Office of Science user facility. DOE supports fundamental research at PNNL in chemistry, physics and materials sciences that are key to materials development. The UW brings complementary facilities and equipment to the partnership, such as the Washington Clean Energy Testbeds and a cryo-electron microscopy facility, as well as expertise in a variety of “big data” research and training endeavors, highly rated research and education programs, and ongoing materials research projects through the National Science Foundation-funded Molecular Engineering Materials Center.

See the original UW News article for additional photos from the event.

UW Molecular Engineering Materials Center established through NSF MRSEC award

The University of Washington is home to a new national center of excellence for research, education, and training in materials science. The UW Molecular Engineering Materials Center is funded by a $15.6 million, six-year grant from the National Science Foundation as part of its highly competitive Materials Research Science and Engineering Center (MRSEC) program. The UW center is a partnership among UW faculty from the College of Arts & Sciences, the College of Engineering, the Clean Energy Institute (CEI) and the Molecular Engineering & Sciences Institute (MolES).

The new center builds on the UW’s record of innovative, collaborative and cross-disciplinary research in the materials sciences, and on a legacy of timely institutional and state investments in materials research at the UW. Initial research will focus on nanocrystals and thin films — toward goals such as developing new materials for applications in clean energy, photonics and quantum computing.

“The primary goal of the UW MRSEC is to empower the next generation of science and engineering leaders,” said center director and UW chemistry professor Daniel Gamelin. “This will involve engaging and supporting students and postdoctoral researchers — and giving them the research and educational experiences, training and cross-disciplinary mentorship that they will need to forge careers on the cutting edge of materials science.”

The center will embark on new research and training endeavors to:

  • Pursue so-called “moonshot” projects, which are research endeavors with potentially high payoff, but are generally beyond the feasibility of smaller research grants awarded to individual professors.
  • Implement new cross-disciplinary training and mentorship programs for doctoral students and postdoctoral researchers, including opportunities to conduct research with the center’s industrial and international partners, and with partners at Pacific Northwest National Laboratory and at other National Laboratories run by the U.S. Department of Energy.
  • Broaden educational and research opportunities for UW students and researchers, including advanced training on new equipment purchased with center funds.
  • Expand outreach and mentorship efforts to high school students from underrepresented minorities to encourage them to pursue science, technology, engineering and math (STEM) education as undergraduates.
  • Implement comprehensive outreach efforts to recruit military veterans at the UW and at local community colleges into research and education for STEM careers.
  • Provide support for additional doctoral and postdoctoral researchers.

The center’s inaugural team of 15 faculty come from a variety of disciplines across engineering and the physical sciences. In addition to their home departments in the College of Engineering and the College of Arts & Sciences, 10 are also faculty members in the CEI and 11 in the MolES. This diverse cohort reflects the center’s goal to foster novel and innovative collaborations across traditionally separate disciplines.

The center will make use of existing research and education space across the UW campus, including in the Molecular Engineering & Sciences Building. The CEI and the MolES, both of which are headquartered in that building, will provide access to equipment for center research and training.

The center’s outreach activities — both within the UW and around the region — emphasize education and training for materials science careers. Each year it will host a Research Experiences for Undergraduates program for students from around the country to conduct research with a UW faculty member during the summer. In addition, center scientists will mentor pre-college students from underrepresented minority groups, providing support and resources to help prepare them for college and encourage them to pursue STEM education. In an entirely new endeavor, the center also will set up programs to engage veterans in center research, very few of whom pursue STEM education and careers.

“With this NSF support, the center will bring new opportunities in STEM education to groups that are underrepresented in STEM careers,” said UW professor of materials science & engineering Christine Luscombe, who is the center’s executive director for education and outreach. “Programs like these are expanding access to science.”

The center will focus on two broad research areas, in nanocrystals and thin films.

The first goal, co-led by Gamelin and Luscombe and including eight initial faculty members, is to pursue new approaches to engineer defects in nanocrystals such as semiconductor quantum dots. Though “defects” often have a negative connotation, in materials science they are opportunities to create substances with novel and technologically attractive properties. Precisely targeted defects or impurities, for example, could make a substance cool down — rather than heat up — when hit by a laser. These new materials could also lead to products such as solar-concentrating window films that absorb photons from sunlight and shunt them to photovoltaic cells for energy conversion.

The center’s other focus is the creation of new ultrathin semiconductor materials with unique properties. This team will include seven initial faculty, and is co-led by associate professor of physics and materials science and engineering Xiaodong Xu and assistant professor of physics and electrical engineering Kai-Mei Fu. This research creates thin sheets of materials — often just one layer of atoms thick — and investigates the unique quantum-mechanical properties revealed when these sheets are layered together. These layered materials could form the basis of new ultrathin semiconductors for applications in clean energy, optoelectronics and other applications. In fact, using this approach, one UW team recently discovered a 2-D magnetic material.

“We chose nanocrystals and ultrathin semiconductors because they promise to yield basic, fundamental and impactful discoveries in materials science,” said Gamelin. “And those advances will fuel new innovations and applications in growing industries — from quantum computing to clean energy.”

Gamelin, Xu, and Fu — along with assistant professor of chemistry Brandi Cossairt and electrical engineering professor Scott Dunham — represented the UW team in Washington, D.C., during the final leg of the multi-stage competition for NSF-MRSEC support. Funding for the UW’s Molecular Engineering Materials Center began September 1. The NSF supports 20 MRSECs across the nation, and the UW’s is one of only two on the West Coast.

For more information, please visit the UW Molecular Engineering Materials Center website:

Story by James Urton, UW News. Additional coverage from GeekWire and the Seattle Times.

Recent work by Alshakim Nelson published in The Economist

Recent work by Assistant Professor Alshakim and colleagues was published on July 6 in the Science & Technology section of The Economist (“Print me a brewery”), as well as in Geekwire and Digital Trends.

Yeast and other microbial species have long been used in a variety of industrial applications, ranging from alcohol fermentation to pharmaceutical and protein production. Fermentation is typically performed as a batch process, wherein the cells and the necessary metabolites are all introduced into a stirred reactor. Professor Nelson and his research team (Dr. Abhijit Saha, Trevor Johnston, and Ryan Shafranek) developed a process to 3D print yeast cells immobilized within a hydrogel lattice, which could be used for fermentation processes. The advantage of this approach is that these yeast-immobilized 3D printed lattices can potentially reduce reactor downtime, minimize product inhibition, and reduce the total footprint of the reactor. The yeast cells are incorporated into the hydrogel under mild conditions while maintaining the viability and metabolic activity of the embedded cells. The Nelson group has shown that these 3D-printed bioreactors are capable of fermenting solutions of glucose into ethanol, continuously, for upwards of three months.

Future work in this area by the Nelson group will include exploring the lifetimes of the bioreactor devices, while also expanding the portfolio of compounds that the reactors can produce, and the species of microbes they can maintain. Eventually, the group would like to implement these printed reactors in continuous fermentation reactors to produce high-value chemical targets.

To learn more about Professor Nelson and his research, visit his faculty page or research group website.

South Asia ICEMR receives 7-year, $9.3M renewal from NIH

Pradipsinh Rathod, left, and Laura Chery, right. Dennis Wise/University of Washington

The National Institutes of Health has renewed a major grant that funds a University of Washington-led research center to understand malaria in India.

The initiative — Malaria Evolution in South Asia (MESA), first funded in 2010 — is one of 10 NIH-supported International Centers of Excellence for Malaria Research, or ICEMRs. The National Institute of Allergy and Infectious Diseases announced that it would provide $9.3 million in funds to the South Asia ICEMR over the next seven years, beginning July 1, 2017.

South Asia sits in the middle of the malaria corridor that cuts from Southeast Asia to Africa.

“India is a country of critical importance for understanding the spread of virulent malaria globally,” said Pradipsinh K. Rathod, a UW professor of chemistry and the director of the Malaria Evolution in South Asia ICEMR. “While most deaths caused by drug-resistant strains of malaria have occurred in Africa, most drug-resistant parasites arise first in Asia.”

Malaria in India remains underappreciated. The country has 1.3 billion people and more than 90% of the population live in areas where there is risk of malaria transmission. India had an estimated 13 million cases of malaria in 2015, according to the World Health Organization. Beyond that, the picture of malaria in India is one of diversity.

“There is enormous variation in the prevalence of malaria around the country — variation in levels of immunity and variation in the species of mosquitoes that spread the disease,” said Laura Chery, the South Asia ICEMR’s associate director. “Most importantly, there is unexpectedly high genetic diversity in malaria parasites that are circulating in India.”

In addition to researchers from the UW, the South Asia ICEMR also includes U.S. scientists from Harvard University, the Fred Hutchinson Cancer Research Center, the Center for Infectious Disease Research and Stanford University. But by far the largest contingent of researchers that make up the center’s efforts are the dozens of scientists, clinicians and field workers at sites across India.

“We have formed wonderful, productive partnerships with hospitals, clinics, government agencies and community members,” said Chery. “Together, we have learned to do advanced science on the ground at clinically important sites.”

Through partnerships with local hospitals and research institutes, the center currently works out of six sites across India. The locations capture the diversity of this massive country: Four sites are in eastern and northeastern India, where malaria is endemic and cases can reach as high as 50 to 100 per 1,000 people. Two other sites are on the west coast, where the prevalence of malaria can be relatively low — fewer than 1 case per 1,000 people. But these sites include urban hospitals that attract and treat large numbers of malaria patients, including migrants from other parts of the country.

“We believe that movement of people within the country can partly explain the complexity of malaria in India,” said Rathod. “However, we do not fully understand the basis for such variations.”

At each site, staff enroll patients to obtain malaria parasite samples, as well as information on each patient’s health history. From on-site laboratories in India, center staff and partners pursue a number of research projects: analyzing parasite samples for signs of drug resistance, understanding the basis for variations in disease presentation, sequencing parasite genomes and determining their genetic relatedness to one another, and testing how well different mosquito species take up various malaria strains.

In addition to setting up complex research infrastructure, in its first seven years the center has made some surprising conclusions about malaria in India. Parasites in India show more genetic diversity than parasites in the rest of the world combined, according to Rathod. As a consequence, some standard laboratory tests for drug resistance, developed elsewhere in the world, do not accurately predict whether Indian parasites will show drug resistance.

Drug resistance is a major concern in malaria. Chloroquine was once an effective drug to fight malaria. But a generation ago, malaria parasites began to evolve resistance to it, rendering it largely ineffective. Today, the drug artemisinin is considered the best treatment against malaria. But artemisinin-resistant strains of malaria already have been identified in Southeast Asia. The Indian government and the South Asia ICEMR are on the lookout for artemisinin resistance among patients in northeastern and eastern India. Beyond that, the South Asia ICEMR is looking for parasites that mutate at extraordinary rates, as seen in Southeast Asia.

“By getting a clearer picture of malaria in India, we’re ‘closing the gap’ on how this complex parasite behaves globally,” Rathod said.

For the 2017-2024 cycle, other South Asia ICEMR project leaders are Neena Valecha, director of the National Institute of Malaria Research in India, and Manoj Duraisingh at Harvard University. Additional U.S.-based senior contributors are Joseph Smith at the Center for Infectious Disease Research, Shripad Tuljapurkar at Stanford University and James Kublin and Holly Janes at the Fred Hutchinson Cancer Research Center. Additional India-based senior contributors are Anup Anvikar at National Institute of Malaria Research; Subrata Baidya at Agartala Government Medical College; D.R. Bhattacharrya and P.K. Mohapatra at Regional Medical Research Centre, NE Region; Edwin Gomes at Goa Medical College & Hospital; Sanjeeb Kakati at Assam Medical College; Ashwani Kumar at National Institute of Malaria Research, Goa Field Unit; Sanjib Mohanty and A.K. Singh at Ispat General Hospital; and Swati Patankar at Indian Institute of Technology Bombay.

For more information about Professor Rathod and his research, please visit his faculty page or the NIH NIAID South Asia ICEMR website.

Story by James Urton, UW News. Additional coverage in the July 2017 Perspectives Newsletter from the College of Arts & Sciences.


Recent work by David Ginger and coworkers published in Nature Materials

Recent work by Professor David Ginger and colleagues was published online on June 19 in Nature Materials. The research was also highlighted in a News & Views article.

Lead author Rajiv Giridharagopal, left, and co-author Lucas Flagg standing next to an atomic force microscope. Dane deQuilettes

The Ginger group, in collaboration with Professor Christine Luscombe (Materials Science & Engineering) and the Clean Energy Institute, has discovered the basic design principles for constructing polymers that can transport both electrons and ions. This is a critical step toward making polymer-based devices at the interface of biology and electronics, such as improved biosensors and bioelectronics implants.

“Most of our technology relies on electronic currents, but biology transduces signals with ions, which are charged atoms or molecules,” said David Ginger, Alvin L. and Verla R. Kwiram Endowed Professor of Chemistry and chief scientist at the UW’s Clean Energy Institute. “If you want to interface electronics and biology, you need a material that effectively communicates across those two realms.”

UW researchers directly measured a thin film made of a single type of conjugated polymer—a conducting plastic—as it interacted with ions and electrons. They show how variations in the polymer layout yielded rigid and non-rigid regions of the film, and that these regions could accommodate electrons or ions—but not both equally. The softer, non-rigid areas were poor electron conductors but could subtly swell to take in ions, while the opposite was true for rigid regions.

The Luscombe group made new P3HT films that had different levels of rigidity based on variations in polymer arrangement. Tests conducted by the Ginger group showed a clear correlation between polymer arrangement and electrochemical properties. The less rigid and more amorphous polymer layouts yielded films that could swell to let in ions, but were poor conductors of electrons. More crystalline polymer arrangements yielded more rigid films that could easily conduct electrons.

Their results demonstrate how critical the polymer synthesis and layout process is to the film’s electronic and ionic conductance properties. Their findings may even point the way forward in creating polymer devices that can balance the demands of electronic transport and ion transport.

“The implication of these findings is that you could conceivably embed a crystalline material—which could transport electrons—within a material that is more amorphous and could transport ions,” said Ginger. “Imagine that you could harness the best of both worlds, so that you could have a material that is able to effectively transport electrons and swell with ion uptake—and then couple the two with one another.”

See the UW News article for expanded coverage.

To learn more about Professor Ginger and his research, please visit his faculty page and research group website.

To learn more about Professor Luscombe and her research, please visit her faculty page and research group website.

To learn more about the UW Clean Energy Institute, please visit the CEI website.


Recent work by Anne McCoy and coworkers published in Science

McCoy 2015 editWater conducts electricity, but the process by which this familiar fluid passes along positive charges has puzzled scientists for decades.

But in a paper published in the Dec. 2 issue of the journal Science, an international team of researchers has finally caught water in the act — showing how water molecules pass along excess charges and, in the process, conduct electricity.

“This fundamental process in chemistry and biology has eluded a firm explanation,” said co-author Anne McCoy, professor of chemistry. “And now we have the missing piece that gives us the bigger picture: how protons essentially ‘move’ through water.”

The team was led by Mark Johnson, senior author and a professor at Yale University. For over a decade, Johnson, McCoy and two co-authors — Professors Kenneth Jordan at the University of Pittsburgh and Knut Asmis at Leipzig University — have collaborated to understand how molecules in complex arrangements pass along charged particles.

Read the full UW News story here. To learn more about Professor McCoy and her research, visit her faculty page and research group website.

Recent work by David Masiello and coworkers published in Nature Photonics

masiello_nature-photonics_squareRecent work by Associate Professor David Masiello and colleagues was highlighted in a November 7 article in Nature Photonics. The research was also highlighted in Chemical & Engineering News and in a News & Views feature article in Nature Photonics.

Measurement of the two distinct components—scattering and absorption—of a single nanoscale object’s optical extinction provides fundamentally important and complementary information on how that object processes light: either scattering it back to the far-field or converting it into internal excitation. Today, various techniques exist to measure the scattering from individual nanoscale objects, all relying on the detection of scattered photons in regions of zero background. Measuring their absorption, however, is much more complicated due to the fundamental inability to detect extremely small reductions in transmission over statistical fluctuations in the number of photons. This means that the spectroscopic signature of the vast majority of molecules—specifically, those that are transformed into dark states through photoreactions—is difficult to access.

To overcome this challenge, researchers in the Masiello group and the Goldsmith group at the University of Wisconsin–Madison devised a new experimental route to measure the absorption spectra of individual, nonemissive nanoscale objects by photothermal contrast in an optical microresonator cavity.

Photothermal spectroscopies function by inferring an object’s absorption from the localized temperature increase and resulting refractive index inhomogeneity produced by the excited object’s nonradiative decay. In their work, the team coupled individual plasmonic nanorods to an ultrahigh-quality optical microresonator cavity and succeeded in determining the nanorod’s absorption spectrum by monitoring the temperature-dependent attometer shifts in the resonance frequency of microresonator’s whispering gallery modes. These exceedingly small but detectable resonance shifts correspond to temperature increases of ~100 nK (measured at room temperature!), making their absorption spectrometer simultaneously one of the world’s best thermometers. Suprisingly, the nanorod’s absorption spectrum revealed a dense array of sharp Fano interferences arising from its interaction with the whispering gallery modes of the microresonator, allowing the team to deeply explore the hybridization of plasmonic and photonic cavity modes.

This collaborative effort brought together the creativity and talents of several graduate students and postdocs in multiple departments between the two institutions. The results were achieved following years of hard work involving both theorists and experimentalists. Future directions will explore the feasibility of this system to serve as a platform for studying quantum physics at room temperature.

To learn more about Professor Masiello and his research, visit his faculty page and research group website.

Nature Methods cover article details refined, accessible technique for expansion microscopy

dividing_finalCongratulations to Assistant Professor Joshua Vaughan and his UW co-workers, whose recent work was featured on the cover of Nature Methods. Their report details the development of a simplified method to “inflate” cellular structures for use in an imaging technique known as expansion microscopy.

Efforts to improve the resolution of cellular structures typically focus on addressing the limitations of microscope hardware. With expansion microscopy, higher resolution is achieved through physical alteration of the specimen. By linking swellable polymers to customized fluorophores, researchers can physically expand the specimen to enable super-resolution microscopy with a conventional laboratory microscope.

As noted in the journal, Vaughan and co-workers have “developed and characterized new methods for linking fluorophores to the polymer that now enable expansion microscopy with conventional fluorescently labeled antibodies and fluorescent proteins.” By simplifying the procedure and expanding fluorophore options, they came up with separate approaches to provide high resolution imaging of individual cells and of tissue slices. In addition to facilitating a range of biological studies, these refinements broadly expand access to the technique, enabling researchers to use a variety of conventional fluorophores and ordinary laboratory microscopes to achieve high resolution cellular imaging.

More information about this work can be found in Nature Methods and in the UW News press release.

For more information about Professor Vaughan and his research, please visit his faculty page and research group website.

Recent work by Jesse Zalatan featured on the cover of Cell

Assistant Professor Jesse Zalatan and co-workers at the UCSF have developed a method to encode complex, synthetic transcriptional regulatory programs using the CRISPR-Cas system. Natural biological systems can switch between different functional or developmental states depending on the particular set of genes being expressed, and the ability to synthetically control gene expression has important implications as both a research tool and as a means to engineer novel cell-based therapeutics and devices.

Zalatan and coworkers designed CRISPR-Cas RNA scaffold molecules that specify both a DNA target and the function to execute at the target, so that sets of RNA scaffolds can be used to generate a synthetic, multigene transcriptional program in eukaryotic cells in which some genes are activated and others are repressed. These types of programs can be used to reprogram complex reaction networks in biological systems, such as metabolic pathways or signaling cascades.

For more information about Professor Zalatan and his research, please visit his faculty page and research group website.